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ORIGINAL ARTICLE
Year : 2013  |  Volume : 15  |  Issue : 2  |  Page : 45-48

Comparison of various methods for detection of AmpC β-lactamase enzyme


Department of Microbiology, Bharati Vidyapeeth University Medical College, Pune, Maharashtra, India

Correspondence Address:
Vrushali Harsh Thakar
Department of Microbiology, Bharati Vidyapeeth University Medical College, Pune, Maharashtra
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0972-1282.124585

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Fifty multidrug-resistant bacterial isolates were tested for extended-spectrum β-lactamase (ESBL) and AmpC β-lactamase enzyme production. Cefoxitin (30 μg) resistance was used as the screening test for AmpC enzyme detection. Phenotypic confirmation was done by conventional three-dimensional enzyme extract test (TDET), AmpC disc test and modified three-dimensional test (MDT). A total of 45 (90%) strains were AmpC positive by the screening test. Out of these 45 strains, 40 strains were positive by AmpC disc test, 39 strains by TDET and 38 strains by MDT. Cefoxitin resistance can be used as the screening test. AmpC disc test is simple to perform and gives rapid result. So, it can be used as a phenotypic method for detection of AmpC enzyme production in resource-limited settings where genotypic detection methods are not available.


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