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Year : 2022  |  Volume : 24  |  Issue : 3  |  Page : 1-7

Introduction to genome sequencing, principles and its applications to a diagnostic medical microbiology laboratory

1 Department of Central Research Laboratory, KIMS Medical College, Bengaluru, Karnataka, India
2 Department of Microbiology, Global Hospitals, Hyderabad, Telangana, India, India

Correspondence Address:
Kadahalli Lingegowda Ravi Kumar
PI - National Centre for Pneumococcal Immunogenicity Evaluation, GHRU, India Unit - Genomic AMR Surveillance CRL, Kempegowda Institute of Medical Sciences, Banashankari 2nd Stage, Bengaluru - 560 070, Karnataka
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/jacm.jacm_14_22

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Microbiology diagnostic laboratory plays a significant role in public health surveillance, outbreak investigation, infection prevention and control strategies. It is moving towards incorporating molecular biology techniques for the surveillance and identification of pathogens causing infectious diseases. Next-generation sequencing (NGS) holds potential for improving clinical and public health microbiology. In addition to identifying pathogens more rapidly and precisely than traditional methods, sequencing technologies can provide new insights into disease transmission, virulence and antimicrobial resistance. NGS has not only reduced the cost of total sequencing but has also introduced versatile applications under one platform. This review will discuss the methods, principles and applications of genome sequencing in microbiology diagnostic laboratories.

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